To make breakthrough progress in the molecular understanding oftranscription and its regulation
disturbances in transcriptional regulation contribute cancer, inflammation, heart disease and metabolic diseases.
Essential to be able to direct this development to form only the wanted type of cells
Gene targeting in ES cell
1. ES cell culture
2. Constructionof targeting vector
3. ES
of protein kinase A by cyclic AMP.
Phosphorylationof enzyme(activity increase or decrease) or phosphorylationof the transcription factor CREB.
Glucagon increases intracellular concentrations of calcium by a mechanism that depends upon activationof protein kinase A.
Increased intracellular calcium reinforce some cyclic AMP mediated actions of glucagon, particularly on glycogenolysis.
Ke
In this preliminary study, the variability of COX-2 overexpression in cervical premalignant lesions was assessed.
NSAIDs and COX-2 inhibitors show anti-cancer activities in many cancer cells.
Aspirin showed strongest inhibitory effects on viability and survival of YD-8 cells.
Aspirin treatment resulted in severe cell shrinkage and nuclear DNA fragmentation in YD-8 cells.
Lipopolysaccharides (LPS), also known as lipoglycans,
are large molecules consisting of a lipid and a polysaccharide
joined by a covalent bond; they are found in the outer
membrane of Gram-negative bacteria, act as endotoxins and
elicit strong immune responses in animals.
LPS is the major component of the outer membrane of
Gram-negative bacteria, contributing greatly to the structu
of free FA or ketone
→ Thus WAT is transformed to Fat burning cells
Expressionof PPARα causes leptin action by up-regulating of carnitine palmitoyl transferase(CPT)-1 and acyl CoA oxidase(ACO) which are target enzyme of FA oxidation
It can be considered on that fact that in the WAT expressionof PPARα is occurred but in the PPARα -/- condition expressionof PPARα mRNA is not occurred
on the enzyme.
Arachidonic acid is initially cyclized and oxidized to the endoperoxide PGG2 at the cyclooxygenase site of the COX and the product then reduced to a second endoperoxide PGH2 at the peroxidase site. Subsequent formationof prostaglandin end products from PGH2 depends on the presence of specific synthases that produce the functionally important prostanoids PGD2, PGE2, PGF2, PGI2 (p
Introduction
BUT, How can we enhance
the Efficiency of Transgene Expression?
Regulationof Transgene Insertion
ZFN : fok1 restrict enzyme + Zinc Finger Protein
Fok1 restrict enzyme : recognition domain andnuclease domain are separated.
Zinc Finger Protein can recognize 9 base pairs.
ZFN can recognize 9 base pairs for each side by dimerization.
Specific sites can be cle
오스트레일리아의 대륙에 처음으로 발을 디딘 사람은 Warramurrungunji라는 여성이다. 그냐는 바다로부터 북오스트레일리아 섬에 나타났고, 내륙으로 향하였으며, 그 과정에서 아이들을 낳고, 각 아이들을 특정 공간에 두었다. 그녀가 들판을 가로질러 이동하며, Warramurrungunji는 각 아이들에게 “나는 너희
1)Concept of Biotope
Biotope
: an area of uniform environmental conditions providing a living place for a specific assemblage of plants and animals
: the concept of habitat as a prerequisite for an organism's existence
Development background
2. History of development
1960s
Discovery of colony-stimulating factors (CSF)
1980s
GM-CSF, M-CSF, G-CSF and multi-CSF
The human G-CSF was cloned
Usage for the treatment at first
1990s
first approval of Filgrastim by the US Food and Drug Administration
Launched Lenograstim
2000s
Launched PEG-filgrastim